Journal: Advanced Healthcare Materials
Article Title: Dual‐Layer Nanoengineered Urinary Catheters for Enhanced Antimicrobial Efficacy and Reduced Cytotoxicity
doi: 10.1002/adhm.202401700
Figure Lengend Snippet: Histological findings in the rabbit penile urethra. a) Representative histological and immunohistochemical images of hematoxylin and eosin (H&E), Masson's trichrome, and terminal deoxynucleotidyl transferase‐mediated dUTP nick and labeling (TUNEL) staining. Moderate to severe inflammatory reactions and hematuria were observed in the narrow urethra of the uncoated and AgNPs groups. Conversely, the Zn/AgNPs group showed an intact epithelial layer and preserved urethral patency. b) Histological results from the catheterized penile urethra in the uncoated, AgNP, and Zn/AgNP groups (Kruskal‐Wallis test; n = 16 per group; error bars showed standard deviations; * p < 0.05 and *** p < 0.001).
Article Snippet: Fetal bovine serum (FBS) was purchased from Gibco, USA; the bladder epithelial cell growth kit (pcs‐420‐042) and bladder epithelial cell basal medium (pcs‐420‐032) from ATCC, USA; 4% paraformaldehyde from Biosesang, Korea; Alexa Fluor 555 Phalloidin (phalloidin) and 4′,6‐diamidino‐2‐phenylindole (DAPI; ProLong Gold antifade reagent with DAPI) from Invitrogen, USA; the D‐Plus CCK cell viability assay kit (CCK‐8 assay kit) from Dongin LS, Korea; potassium chloride for rabbit sacrifice from JW Pharm, Korea; and artificial urine (DIN EN 1616:1999 Artificial Urine, BZ102S; Biochemazone) from Leduc, Canada.
Techniques: Immunohistochemical staining, Labeling, TUNEL Assay, Staining